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Directed Evolution of Orthogonal Ligand Specificity in a Single Scaffold
Author(s) -
McLachlan Michael J.,
Chockalingam Karuppiah,
Lai Ka Chun,
Zhao Huimin
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200903413
Subject(s) - mcherry , ligand (biochemistry) , directed evolution , scaffold , green fluorescent protein , computer science , scaffold protein , computational biology , mutant , receptor , biology , genetics , gene , programming language , signal transduction
Two highly sensitive ligand–receptor pairs created from a single protein scaffold were fully orthogonal both to the native ligand–receptor pair and to one another. The wild‐type receptor and two mutants were activated specifically by their respective ligands (represented by red shapes in the picture) when used to control the expression of green fluorescent protein (top row), mCherry (middle row), and yellow fluorescent protein (bottom row) in yeast.