Premium
DNA Origami as a Nanoscopic Ruler for Super‐Resolution Microscopy
Author(s) -
Steinhauer Christian,
Jungmann Ralf,
Sobey Thomas L.,
Simmel Friedrich C.,
Tinnefeld Philip
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200903308
Subject(s) - nanoscopic scale , dna origami , nanotechnology , resolution (logic) , dna nanotechnology , nanostructure , microscopy , ruler , atomic force microscopy , dna , computer science , materials science , chemistry , physics , optics , artificial intelligence , biochemistry , quantum mechanics
Resolving the distances : Rectangular DNA origami labeled with fluorophores at specific positions has been used as a nanoscopic ruler. Super‐resolution microscopy based on the subsequent localization of single molecules enables two fluorophores at a distance of about 90 nm to be optically resolved. This combination of subdiffraction imaging and DNA nanotechnology opens up new avenues for studying nanostructures and their dynamics.