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A High‐Resolution Interaction Map of Three Transcriptional Activation Domains with a Key Coactivator from Photo‐Cross‐Linking and Multiplexed Mass Spectrometry
Author(s) -
Majmudar Chinmay Y.,
Wang Bo,
Lum Jenifer K.,
Håkansson Kristina,
Mapp Anna K.
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200902669
Subject(s) - coactivator , chemistry , computational biology , mass spectrometry , yeast , biology , biochemistry , transcription factor , chromatography , gene
Binding with distinction : Photo‐cross‐linking followed by the complementary use of ESI and MALDI mass spectrometric techniques yielded the interaction sites of three prototypical amphipathic transcriptional activators (represented by different‐colored helices in the picture) with the coactivator Med15. Investigation of the functional relevance of these sites in yeast revealed overlapping yet distinct binding patterns for the activators investigated.