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Secondary‐Structure‐Inducible Ligand Fluorescence Coupled with PCR
Author(s) -
Takei Fumie,
Igarashi Masako,
Hagihara Masaki,
Oka Yoshimi,
Soya Yoshihiro,
Nakatani Kazuhiko
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200902449
Subject(s) - fluorescence , primer (cosmetics) , computational biology , bulge , biology , chemistry , biophysics , physics , optics , stars , organic chemistry , astronomy
Not second fiddle : Hairpin secondary structures at the 5′ end of the PCR primer are transformed into a double‐stranded form as the PCR proceeds. A fluorescent molecule (DANP) can bind to the single cytosine bulge (C‐bulge) in the hairpin structure and emit characteristic fluorescence. When the PCR primer labeled with a C‐bulge hairpin tag is used in the presence of DANP, fluorescence from the DANP‐C‐bulge complex decreases as the PCR proceeds.