Premium
Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters
Author(s) -
Luković Elvedin,
Vogel Taylor Elizabeth,
Imperiali Barbara
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200902374
Subject(s) - kinase , chemistry , docking (animal) , recombinant dna , mapk/erk pathway , selectivity , enzyme , high throughput screening , biochemistry , biophysics , microbiology and biotechnology , combinatorial chemistry , biology , catalysis , gene , medicine , nursing
The best of both worlds : Combining a recombinant docking domain (see picture, blue ribbon) with a chemical sensing module (green hexagons) yields a highly selective ERK sensor. This probe exclusively monitors ERK1/2 activity in unfractionated cell lysates without any off‐target kinase inhibitors. Good selectivity and biophysical parameters allow high‐throughput analysis of ERK1/2 activity without time‐consuming enzyme purification.