Deglycosylation of Glucose Oxidase for Direct and Efficient Glucose Electrooxidation on a Glassy Carbon Electrode | Zendy

Courjean Olivier | Zendy; Gao Feng | Zendy; Mano Nicolas | Zendy

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Deglycosylation of Glucose Oxidase for Direct and Efficient Glucose Electrooxidation on a Glassy Carbon Electrode

Author(s) -

Courjean Olivier,

Gao Feng,

Mano Nicolas

Publication year - 2009

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.200902191

Subject(s) - glucose oxidase , electrode , glassy carbon , chemistry , carbon fibers , enzyme , monolayer , inorganic chemistry , electrochemistry , materials science , biochemistry , cyclic voltammetry , composite material , composite number

Down to the bare bones : When a glucose oxidase (GOx, left) was deglycosylated, the modified enzyme (right) immobilized on a vitreous carbon electrode came into closer electrical contact with the surface than the native enzyme. Glucose was electrooxidized directly on a monolayer of deglycosylated GOx on a carbon electrode at the unprecedented reducing potential of −200 mV versus Ag/AgCl and with a current density of 235 μA cm −2 .

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