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Deglycosylation of Glucose Oxidase for Direct and Efficient Glucose Electrooxidation on a Glassy Carbon Electrode
Author(s) -
Courjean Olivier,
Gao Feng,
Mano Nicolas
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200902191
Subject(s) - glucose oxidase , electrode , glassy carbon , chemistry , carbon fibers , enzyme , monolayer , inorganic chemistry , electrochemistry , materials science , biochemistry , cyclic voltammetry , composite material , composite number
Down to the bare bones : When a glucose oxidase (GOx, left) was deglycosylated, the modified enzyme (right) immobilized on a vitreous carbon electrode came into closer electrical contact with the surface than the native enzyme. Glucose was electrooxidized directly on a monolayer of deglycosylated GOx on a carbon electrode at the unprecedented reducing potential of −200 mV versus Ag/AgCl and with a current density of 235 μA cm −2 .