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Human Telomeric Quadruplex Conformations Studied by Pulsed EPR
Author(s) -
Singh Vijay,
Azarkh Mykhailo,
Exner Thomas E.,
Hartig Jörg S.,
Drescher Malte
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200902146
Subject(s) - g quadruplex , electron paramagnetic resonance , guanosine , oligonucleotide , telomere , computational biology , computer science , chemistry , biology , crystallography , gene , physics , biochemistry , nuclear magnetic resonance , dna
Distance learning : Under physiological conditions telomeric sequences adopt different quadruplex topologies, although the exact nature of these species is currently debated. Double spin‐labeled oligonucleotides consisting of guanosine‐rich telomeric repeats are synthesized and characterized by pulsed EPR techniques. In solution in the presence of K + ions the propeller and basket quadruplex conformations co‐exist as a 1:1 mixture.