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Chemoselective Staudinger‐Phosphite Reaction of Azides for the Phosphorylation of Proteins
Author(s) -
Serwa Remigiusz,
Wilkening Ina,
Del Signore Giuseppe,
Mühlberg Michaela,
Claußnitzer Iris,
Weise Christoph,
Gerrits Michael,
Hackenberger Christian P. R.
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200902118
Subject(s) - staudinger reaction , chemistry , phosphorylation , toolbox , combinatorial chemistry , aqueous solution , organic chemistry , biochemistry , computer science , programming language
Extending the toolbox : The title reaction was identified as a chemoselective means to modify azides in peptides and proteins in high yields at room temperature in various solvents including aqueous buffers at physiological pH. In combination with nonnatural protein translation the Staudinger‐phosphite reaction allows the site‐specific incorporation of phosphorylated Tyr analogues in proteins.