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Ultrasensitive and Selective Colorimetric DNA Detection by Nicking Endonuclease Assisted Nanoparticle Amplification
Author(s) -
Xu Wei,
Xue Xuejia,
Li Tianhu,
Zeng Huaqiang,
Liu Xiaogang
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200901772
Subject(s) - endonuclease , oligonucleotide , dna , computational biology , detection limit , microbiology and biotechnology , restriction enzyme , computer science , biology , chemistry , genetics , chromatography
One–two punch : Single stranded DNA sequences can be detected by nicking endonuclease assisted nanoparticle amplification (see picture). The detection system offers a colorimetric detection limit of 0.5 fmol within hours for selected oligonucleotides. Detection of DNA sequences with a single base mismatch or different lengths is also demonstrated.