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Segmental Isotopic Labeling of a Central Domain in a Multidomain Protein by Protein Trans ‐Splicing Using Only One Robust DnaE Intein
Author(s) -
Busche Alena E. L.,
Aranko A. Sesilja,
TalebzadehFarooji Mehdi,
Bernhard Frank,
Dötsch Volker,
Iwaï Hideo
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200901488
Subject(s) - intein , domain (mathematical analysis) , protein splicing , rna splicing , trans splicing , chemistry , computational biology , protein domain , biology , biochemistry , rna , gene , mathematical analysis , mathematics
A split intein saves nine : A naturally split intein is used to create an efficient three‐way ligation method that does not require any refolding steps. This method enables the selective labeling of a central domain within a three‐domain protein, with NMR active isotopes (see picture) allowing domain–domain interactions in multidomain proteins to be investigated.