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Taking Fingerprints of DNA Polymerases: Multiplex Enzyme Profiling on DNA Arrays
Author(s) -
Kranaster Ramon,
Marx Andreas
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200900953
Subject(s) - dna profiling , multiplex , dna , dna polymerase , computational biology , multiplex polymerase chain reaction , polymerase , primase , biology , genetics , microbiology and biotechnology , polymerase chain reaction , gene , reverse transcriptase
Abstract A novel screening approach based on an oligonucleotide‐addressing enzyme assay enables multiplexed simultaneous profiling of DNA polymerases in nanoliter volumes in terms of their different properties. This approach was used to identify enzymes with altered properties out of a library of protein mutants.