A Facile System for Encoding Unnatural Amino Acids in Mammalian Cells | Zendy

Chen Peng R. | Zendy; Groff Dan | Zendy; Guo Jiantao | Zendy; Ou Weijia | Zendy; Cellitti Susan | Zendy; Geierstanger Bernhard H. | Zendy; Schultz Peter G. | Zendy

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A Facile System for Encoding Unnatural Amino Acids in Mammalian Cells

Author(s) -

Chen Peng R.,

Groff Dan,

Guo Jiantao,

Ou Weijia,

Cellitti Susan,

Geierstanger Bernhard H.,

Schultz Peter G.

Publication year - 2009

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.200900683

Subject(s) - transfer rna , aminoacyl trna synthetase , amino acid , genetic code , amino acyl trna synthetases , mutant , biochemistry , lysine , nonsense mutation , biology , chemistry , mutation , rna , gene , missense mutation

A shuttle system has been developed to genetically encode unnatural amino acids in mammalian cells using aminoacyl‐tRNA synthetases (aaRSs) evolved in E. coli. A pyrrolysyl‐tRNA synthetase (PylRS) mutant was evolved in E. coli that selectively aminoacylates a cognate nonsense suppressor tRNA with a photocaged lysine derivative. Transfer of this orthogonal tRNA–aaRS pair into mammalian cells made possible the selective incorporation of this unnatural amino acid into proteins.

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