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A Facile System for Encoding Unnatural Amino Acids in Mammalian Cells
Author(s) -
Chen Peng R.,
Groff Dan,
Guo Jiantao,
Ou Weijia,
Cellitti Susan,
Geierstanger Bernhard H.,
Schultz Peter G.
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200900683
Subject(s) - transfer rna , aminoacyl trna synthetase , amino acid , genetic code , amino acyl trna synthetases , mutant , biochemistry , lysine , nonsense mutation , biology , chemistry , mutation , rna , gene , missense mutation
A shuttle system has been developed to genetically encode unnatural amino acids in mammalian cells using aminoacyl‐tRNA synthetases (aaRSs) evolved in E. coli. A pyrrolysyl‐tRNA synthetase (PylRS) mutant was evolved in E. coli that selectively aminoacylates a cognate nonsense suppressor tRNA with a photocaged lysine derivative. Transfer of this orthogonal tRNA–aaRS pair into mammalian cells made possible the selective incorporation of this unnatural amino acid into proteins.