Premium
Mechanical Nanosensor Based on FRET within a Thermosome: Damage‐Reporting Polymeric Materials
Author(s) -
Bruns Nico,
Pustelny Katarzyna,
Bergeron Lisa M.,
Whitehead Timothy A.,
Clark Douglas S.
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200900554
Subject(s) - förster resonance energy transfer , fluorophore , nanosensor , biophysics , polymer , fluorescence , materials science , chemistry , nanotechnology , composite material , biology , physics , optics
Under stress : Changes in stress of the polymer matrix in a protein–polymer hybrid material result in changes of conformation of the protein complex, thus resulting in a damage‐reporting material (see picture). The reporter is an engineered chaperonin that covalently entraps a pair of fluorescent proteins. Deformation of the chaperonin leads to a change in fluorophore distance and a change in the fluorescene resonance energy transfer (FRET) signal.