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Iterative In Situ Click Chemistry Creates Antibody‐like Protein‐Capture Agents
Author(s) -
Agnew Heather D.,
Rohde Rosemary D.,
Millward Steven W.,
Nag Arundhati,
Yeo WoonSeok,
Hein Jason E.,
Pitram Suresh M.,
Tariq Abdul Ahad,
Burns Vanessa M.,
Krom Russell J.,
Fokin Valery V.,
Sharpless K. Barry,
Heath James R.
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200900488
Subject(s) - click chemistry , in situ , combinatorial chemistry , chemistry , cycloaddition , ligand (biochemistry) , peptide , fragment (logic) , nanotechnology , computer science , catalysis , biochemistry , organic chemistry , materials science , receptor , algorithm
Special agents for protein capture : Iterative in situ click chemistry (see scheme for the tertiary ligand screen) and the one‐bead–one‐compound method for the creation of a peptide library enable the fragment‐based assembly of selective high‐affinity protein‐capture agents. The resulting ligands are water‐soluble and stable chemically, biochemically, and thermally. They can be produced in gram quantities through copper(I)‐catalyzed cycloaddition.