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Fluorescence Detection of Single‐Nucleotide Polymorphisms with a Single, Self‐Complementary, Triple‐Stem DNA Probe
Author(s) -
Xiao Yi,
Plakos Kory J. I.,
Lou Xinhui,
White Ryan J.,
Qian Jiangrong,
Plaxco Kevin W.,
Soh H. Tom
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200900369
Subject(s) - fluorescence , fluorophore , single nucleotide polymorphism , dna , nucleotide , chemistry , biophysics , microbiology and biotechnology , genetics , biology , gene , physics , genotype , optics
Singled out for its singularity : In a single‐step, single‐component, fluorescence‐based method for the detection of single‐nucleotide polymorphisms at room temperature, the sensor is comprised of a single, self‐complementary DNA strand that forms a triple‐stem structure. The large conformational change that occurs upon binding to perfectly matched (PM) targets results in a significant increase in fluorescence (see picture; F=fluorophore, Q=quencher).