Fluorescence Detection of Single‐Nucleotide Polymorphisms with a Single, Self‐Complementary, Triple‐Stem DNA Probe | Zendy

Xiao Yi | Zendy; Plakos Kory J. I. | Zendy; Lou Xinhui | Zendy; White Ryan J. | Zendy; Qian Jiangrong | Zendy; Plaxco Kevin W. | Zendy; Soh H. Tom | Zendy

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Fluorescence Detection of Single‐Nucleotide Polymorphisms with a Single, Self‐Complementary, Triple‐Stem DNA Probe

Author(s) -

Xiao Yi,

Plakos Kory J. I.,

Lou Xinhui,

White Ryan J.,

Qian Jiangrong,

Plaxco Kevin W.,

Soh H. Tom

Publication year - 2009

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.200900369

Subject(s) - fluorescence , fluorophore , single nucleotide polymorphism , dna , nucleotide , chemistry , biophysics , microbiology and biotechnology , genetics , biology , gene , physics , genotype , optics

Singled out for its singularity : In a single‐step, single‐component, fluorescence‐based method for the detection of single‐nucleotide polymorphisms at room temperature, the sensor is comprised of a single, self‐complementary DNA strand that forms a triple‐stem structure. The large conformational change that occurs upon binding to perfectly matched (PM) targets results in a significant increase in fluorescence (see picture; F=fluorophore, Q=quencher).

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