Premium
Click Chemistry for the Identification of G‐Quadruplex Structures: Discovery of a DNA–RNA G‐Quadruplex
Author(s) -
Xu Yan,
Suzuki Yuta,
Komiyama Makoto
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200806306
Subject(s) - click chemistry , g quadruplex , rna , dna , cycloaddition , azide , chemistry , combinatorial chemistry , computational biology , stereochemistry , biology , biochemistry , catalysis , organic chemistry , gene
A trap that closes with a “click” : The copper‐catalyzed azide–alkyne cycloaddition can occur in different G‐quadruplex structures (see scheme). The species trapped by the click reaction can then be separated and analyzed. By using this approach, a DNA–RNA hybrid‐type G‐quadruplex structure formed by human telomeric DNA and RNA sequences was detected.