Colorimetric Sensing by Using Allosteric‐DNAzyme‐Coupled Rolling Circle Amplification and a Peptide Nucleic Acid–Organic Dye Probe | Zendy

Ali M. Monsur | Zendy; Li Yingfu | Zendy

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Colorimetric Sensing by Using Allosteric‐DNAzyme‐Coupled Rolling Circle Amplification and a Peptide Nucleic Acid–Organic Dye Probe

Author(s) -

Ali M. Monsur,

Li Yingfu

Publication year - 2009

Publication title -

angewandte chemie international edition

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.831

H-Index - 550

eISSN - 1521-3773

pISSN - 1433-7851

DOI - 10.1002/anie.200805966

Subject(s) - deoxyribozyme , peptide nucleic acid , allosteric regulation , naked eye , nucleic acid , rolling circle replication , chemistry , dna , duplex (building) , molecule , combinatorial chemistry , rna , biophysics , nanotechnology , biochemistry , polymerase , detection limit , biology , receptor , materials science , organic chemistry , chromatography , gene

Target detection by the naked eye : The action of an RNA‐cleaving allosteric DNAzyme in response to ligand binding was coupled to a rolling circle amplification process to generate long single‐stranded DNA molecules for colorimetric sensing (see scheme). Upon hybridization of the resulting DNA with a complementary PNA sequence in the presence of a duplex‐binding dye, the color of the dye changed from blue to purple.

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