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Electrochemical Gene‐Function Analysis for Single Cells with Addressable Microelectrode/Microwell Arrays
Author(s) -
Lin Zhenyu,
Takahashi Yasufumi,
Murata Tatsuya,
Takeda Michiaki,
Ino Kosuke,
Shiku Hitoshi,
Matsue Tomokazu
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200805743
Subject(s) - microelectrode , alkaline phosphatase , hela , electrochemistry , electrode , transfection , enzyme , chemistry , plasmid , cell , microbiology and biotechnology , gene , biophysics , biology , biochemistry
To each his own : An addressable electrochemical device consisting of orthogonally arranged rows and columns of electrodes has been constructed to monitor protein expression in genetically engineered cells at the single‐cell level. The response based on redox cycling reflected the different expression levels of the enzyme from individual HeLa cells transfected with a plasmid vector including secreted alkaline phosphatase.