Premium
Efficient Replication Bypass of Size‐Expanded DNA Base Pairs in Bacterial Cells
Author(s) -
Delaney James C.,
Gao Jianmin,
Liu Haibo,
Shrivastav Nidhi,
Essigmann John M.,
Kool Eric T.
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200805683
Subject(s) - encode , base pair , dna replication , dna , replication (statistics) , biology , dna polymerase , control of chromosome duplication , genetics , computational biology , gene , virology
Abstract Supersize me! Size‐expanded DNA bases (xDNA) are able to encode natural DNA sequences in replication. In vitro experiments with a DNA polymerase show nucleotide incorporation opposite the xDNA bases with correct pairing. In vivo experiments using E. coli show that two xDNA bases (xA and xC, see picture) encode the correct replication partners.