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Selective Fluorescence Labeling of Lipids in Living Cells
Author(s) -
Neef Anne B.,
Schultz Carsten
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200805507
Subject(s) - click chemistry , phosphatidic acid , alkyne , cycloaddition , chemistry , fluorescence , membrane , azide , biochemistry , combinatorial chemistry , phospholipid , organic chemistry , catalysis , physics , quantum mechanics
Click chemistry in vivo : Three phosphatidic acid derivatives with alkyne groups in their fatty acid chains were synthesized and incorporated into mammalian cell membranes. Copper(I)‐catalyzed and strain‐promoted azide–alkyne cycloaddition reactions were used for their visualization (see schematic representation and fluorescence microscopic image).