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Artificial Ribozyme Switches Containing Natural Riboswitch Aptamer Domains
Author(s) -
Wieland Markus,
Benz Armin,
Klauser Benedikt,
Hartig Jörg S.
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200805311
Subject(s) - riboswitch , aptamer , ribozyme , rna , computational biology , mammalian cpeb3 ribozyme , ligase ribozyme , synthetic biology , thiamine pyrophosphate , biology , chemistry , non coding rna , microbiology and biotechnology , gene , biophysics , biochemistry , genetics , enzyme , cofactor
RNA Lego : The use of natural riboswitch aptamers in synthetic RNA switches (see picture) should broaden the scope of artificial RNA regulators dramatically. It is shown that thiamine pyrophosphate (TPP) aptamers can be used in engineered devices as very sensitive switches of gene expression in unmodified organisms. The approach demonstrates that intrinsic metabolites can be utilized as external effectors of cellular functions.