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Engineering A Uranyl‐Specific Binding Protein from NikR
Author(s) -
Wegner Seraphine V.,
Boyaci Hande,
Chen Hao,
Jensen Mark P.,
He Chuan
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200805262
Subject(s) - uranyl , chemistry , dissociation constant , dna , dissociation (chemistry) , dna binding protein , crystallography , biochemistry , gene , transcription factor , organic chemistry , ion , receptor
Abstract A new pick‐up line : The first uranyl‐selective DNA‐binding protein is designed using the E. coli nickel(II)‐responsive protein NikR as the template. The resulting NikR′ protein binds uranyl (see picture) with a dissociation constant K d =53 n M and selectively binds to DNA in the presence of uranyl.