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Relative Orientation of Rigid Nitroxides by PELDOR: Beyond Distance Measurements in Nucleic Acids
Author(s) -
Schiemann Olav,
Cekan Pavol,
Margraf Dominik,
Prisner Thomas F.,
Sigurdsson Snorri Th.
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200805152
Subject(s) - nitroxide mediated radical polymerization , orientation (vector space) , spin label , spin (aerodynamics) , site directed spin labeling , position (finance) , chemistry , nucleic acid , nuclear magnetic resonance , physics , molecular physics , electron paramagnetic resonance , geometry , mathematics , monomer , polymer , biochemistry , radical polymerization , thermodynamics , finance , economics
Show me your angle : Incorporation of the rigid spin label Ç allows determination of both distance and orientation of two nitroxide spin labels in DNA by PELDOR experiments at common X‐band frequencies. The orientational information is obtained by varying the position of the detection pulses over the nitroxide spectrum. Simulation of the set of time traces yields very precise distances and angles.