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Triggered Enzymatic Degradation of DNA within Selectively Permeable Polymer Capsule Microreactors
Author(s) -
Price Andrew D.,
Zelikin Alexander N.,
Wang Yajun,
Caruso Frank
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200804763
Subject(s) - microreactor , capsule , nucleic acid , dna , polymer , chemistry , degradation (telecommunications) , substrate (aquarium) , polymer degradation , biophysics , biochemistry , biology , organic chemistry , catalysis , computer science , telecommunications , ecology , botany
Thinking in compartments is back! The degradation of a fluorescently labeled nucleic acid substrate within a polymer capsule by coencapsulated DNase I was initiated by activation of the enzyme with a chemical trigger. The degraded DNA diffused from the capsule (see picture). The semipermeable capsule microreactors enable external reaction control, high‐throughput tracking of reaction progression, and continuous modification of the DNA cargo.