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Semisynthesis of a Homogeneous Glycoprotein Enzyme: Ribonuclease C: Part 2
Author(s) -
Piontek Christian,
Varón Silva Daniel,
Heinlein Christian,
Pöhner Claudia,
Mezzato Stefano,
Ring Petra,
Martin Andreas,
Schmid Franz Xaver,
Unverzagt Carlo
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200804735
Subject(s) - semisynthesis , ribonuclease , glycoprotein , rnase p , glycan , biochemistry , enzyme , chemistry , cleave , rna , gene
Active RNase glycoprotein from three pieces : The glycoprotein enzyme ribonuclease C, which contains a complex saccharide N‐glycan, was synthesized by sequential native chemical ligation. An optimized ligation and isolation protocol allowed the efficient assembly and refolding of the 124 amino acid enzyme.