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Semisynthesis of a Homogeneous Glycoprotein Enzyme: Ribonuclease C: Part 1
Author(s) -
Piontek Christian,
Ring Petra,
Harjes Olaf,
Heinlein Christian,
Mezzato Stefano,
Lombaelson,
Pöhner Claudia,
Püttner Markus,
Varón Silva Daniel,
Martin Andreas,
Schmid Franz Xaver,
Unverzagt Carlo
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200804734
Subject(s) - semisynthesis , ribonuclease , glycoprotein , homogeneous , enzyme , biochemistry , chemistry , microbiology and biotechnology , biology , rna , mathematics , gene , combinatorics
Seven in one blow : The efficient formation of mixed disulfides on the thiol‐rich fusion protein A followed by subsequent intein cleavage gave the fragment B with all seven cysteines protected against oxidation. The native chemical ligation of B with synthetic glycopeptide thioesters provides glycoproteins.