Premium
Ammosamides A and B Target Myosin
Author(s) -
Hughes Chambers C.,
MacMillan John B.,
Gaudêncio Susana P.,
Fenical William,
La Clair James J.
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200804107
Subject(s) - myosin , fluorescence , fluorescence microscope , construct (python library) , chemistry , computational biology , biochemistry , information retrieval , microbiology and biotechnology , computer science , biology , physics , quantum mechanics , programming language
The protein target of the ammosamides, cytotoxic natural products from a marine‐derived actinomycete, has been elucidated. An immunoaffinity fluorescent tag was used to construct a fluorescent molecular probe (see structure). First, uptake and localization of the probe in cells was visualized with fluorescence microscopy. The probe was then used to co‐immunoprecipitate proteins that bound to the ammosamide core. Myosin was thus identified as a target.