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Affinity‐Based Labeling of Cytohesins with a Bifunctional SecinH3 Photoaffinity Probe
Author(s) -
Bi Xihe,
Schmitz Anton,
Hayallah Alaa M.,
Song JinNa,
Famulok Michael
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200803962
Subject(s) - bifunctional , photoaffinity labeling , proteome , gtpase , guanine nucleotide exchange factor , chemistry , computational biology , profiling (computer programming) , nat , binding site , biochemistry , computer science , biology , programming language , catalysis , computer network
The specific binding of the cytohesin inhibitor SecinH3 to Sec7 domains of cytohesins was shown by the use of a bifunctional photoaffinity probe (see structure) on guanine nucleotide exchange factors (GEFs) and GTPases. This probe should be useful for the proteome‐wide profiling of cytohesin complexes and the identification of the binding site.