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Mass Spectrometry Signal Amplification Method for Attomolar Detection of Antigens Using Small‐Molecule‐Tagged Gold Microparticles
Author(s) -
Lee Jung Rok,
Lee Juhee,
Kim Sang Kyung,
Kim Kwang Pyo,
Park Hyung Soon,
Yeo WoonSeok
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200803893
Subject(s) - mass spectrometry , detection limit , chemistry , chromatography , matrix (chemical analysis) , analytical chemistry (journal) , nanotechnology , materials science
Small but more than enough : Proteins are detected in solution without additional amplification or labeling steps by using a small molecule as a reporter of the protein (see picture, Am‐tag=amplification tag). The combination of self‐assembled monolayers on gold with matrix‐free laser desorption/ionization time‐of‐flight mass spectrometry offers ultrahigh sensitivity with a detection limit of several attomoles.