Premium
Formal Asymmetric Biocatalytic Reductive Amination
Author(s) -
Koszelewski Dominik,
Lavandera Iván,
Clay Dorina,
Guebitz Georg M.,
Rozzell David,
Kroutil Wolfgang
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200803763
Subject(s) - reductive amination , amination , formate dehydrogenase , formate , chemistry , biocatalysis , amine gas treating , stereoselectivity , transaminase , combinatorial chemistry , cofactor , ketone , ammonia , organic chemistry , enzyme , catalysis , reaction mechanism
All for one : A combination of three biocatalysts (ω‐transaminase, alanine dehydrogenase, and an enzyme such as formate dehydrogenase for cofactor recycling) catalyze a cascade to achieve the asymmetric transformation of a ketone into a primary α‐chiral unprotected amine through a formal stereoselective reductive amination (see scheme). Only ammonia and the reducing agent (formate) are consumed during this reaction.