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Self‐Assembly of Antibodies by Chemical Induction
Author(s) -
Li Qing,
Hapka David,
Chen Hua,
Vallera Daniel A.,
Wagner Carston R.
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200803507
Subject(s) - dihydrofolate reductase , recombinant dna , antibody , trimethoprim , chemistry , monomer , antibiotics , biochemistry , stereochemistry , biology , enzyme , immunology , organic chemistry , gene , polymer
Getting together and breaking up : Circular arrays of recombinant antibodies can be produced reversibly by chemically controlled protein macrocyclization. In the presence of bismethotrexate (bis‐MTX), dimeric dihydrofolate reductase fusion protein (DHFR) with an anti‐CD3 single‐chain variable region (scFv) self‐assembled spontaneously into diabodies, which were disassembled into monomers with an excess of the antibiotic trimethoprim (see picture).