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Peptide Fragmentation Assisted by Surfaces Treated with a Low‐Temperature Plasma in NanoESI
Author(s) -
Xia Yu,
Ouyang Zheng,
Cooks R. Graham
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200803477
Subject(s) - fragmentation (computing) , plasma , peptide , chemistry , chromatography , biophysics , computer science , physics , biochemistry , biology , nuclear physics , operating system
A softy toughens up : When a nanoelectrospray (nanoESI) emitter was exposed first to a helium plasma (see illustration of experimental setup), ESI, a widely accepted “soft” ionization technique, caused significant and useful peptide fragmentation. The appearance of abundant peptide‐fragment ions in the mass spectrum was ascribed to electrolyte release into the solution. Labile phosphate groups on the peptide were preserved during fragmentation.