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A Ratiometric Fluorescent Probe Based on FRET for Imaging Hg 2+ Ions in Living Cells
Author(s) -
Zhang Xiaolin,
Xiao Yi,
Qian Xuhong
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200803246
Subject(s) - förster resonance energy transfer , fluorescence , rhodamine , ion , chemistry , photochemistry , energy transfer , mercury (programming language) , analytical chemistry (journal) , optics , physics , chemical physics , organic chemistry , chromatography , computer science , programming language
Mercury rising : A FRET‐based ratiometric probe (see picture; FRET=fluorescence resonance energy transfer) can selectively detect Hg 2+ ions on the parts‐per‐billion scale under physiological conditions. The probe undergoes a clear Hg 2+ ‐induced change in the intensity ratio of the two well‐separated and equally intense emission bands of dipyrrometheneboron difluoride and rhodamine. Experiments show that the probe can be used in living cells.