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A Genetically Encoded Boronate‐Containing Amino Acid
Author(s) -
Brustad Eric,
Bushey Mark L.,
Lee Jae Wook,
Groff Dan,
Liu Wenshe,
Schultz Peter G.
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200803240
Subject(s) - boronic acid , amino acid , biochemistry , chemistry , transfer rna , combinatorial chemistry , computational biology , biology , gene , rna
A biological boronate : An orthogonal tRNA/aminoacyl‐tRNA synthetase pair has been evolved for the genetic incorporation of a boronic acid into proteins. This amino acid has been used to purify proteins in a one‐step scarless purification procedure as well as for the site‐specific labeling of proteins using various boronic acid chemistries.