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Artificial Enzymes Made to Order: Combination of Computational Design and Directed Evolution
Author(s) -
Ward Thomas R.
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200802865
Subject(s) - order (exchange) , directed evolution , computer science , directed molecular evolution , computational biology , biology , genetics , economics , gene , mutant , finance
Intelligent design : The combination of computational design and directed evolution has allowed the creation and optimization of an artificial enzyme starting from a catalytically inactive protein scaffold. In this approach, glutamate, tryptophane, and lysine residues were introduced into a TIM barrel fold to yield an artificial enzyme for the Kemp elimination that was fine‐tuned by directed evolution ( k cat / K m =2590 M −1 s −1 , see picture).