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Monitoring Catalysis of the Membrane‐Bound Hydrogenase from Ralstonia eutropha H16 by Surface‐Enhanced IR Absorption Spectroscopy
Author(s) -
Wisitruangsakul Nattawadee,
Lenz Oliver,
Ludwig Marcus,
Friedrich Bärbel,
Lendzian Friedhelm,
Hildebrandt Peter,
Zebger Ingo
Publication year - 2009
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200802633
Subject(s) - ralstonia , hydrogenase , catalysis , nitrilotriacetic acid , chemistry , photochemistry , infrared spectroscopy , absorption spectroscopy , nickel , redox , inorganic chemistry , spectroscopy , organic chemistry , enzyme , physics , chelation , quantum mechanics
Immobilized biocatalyst : The oxygen‐tolerant, membrane‐bound hydrogenase of Ralstonia eutropha H16 is immobilized by a His‐tag (see picture; green) onto a gold surface (yellow) modified with nickel–nitrilotriacetic acid (red/black). Catalytic activity towards hydrogen is investigated by surface‐enhanced infrared absorption (SEIRA) spectroscopy. Switching redox states and related structural changes of the Ni–Fe active site are followed by the CO and CN − ligand stretching modes.