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DNA‐Coated AFM Cantilevers for the Investigation of Cell Adhesion and the Patterning of Live Cells
Author(s) -
Hsiao Sonny C.,
Crow Ailey K.,
Lam Wilbur A.,
Bertozzi Carolyn R.,
Fletcher Daniel A.,
Francis Matthew B.
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200802525
Subject(s) - cantilever , adhesion , atomic force microscopy , nanotechnology , dna , cell mechanics , membrane , materials science , biophysics , computer science , chemistry , microbiology and biotechnology , cell , biology , composite material , biochemistry , cytoskeleton
Get a grip : Measurement of receptor adhesion strength requires the precise manipulation of single cells on a contact surface. To attach live cells to a moveable probe, DNA sequences complementary to strands displayed on the plasma membrane are introduced onto AFM cantilevers (see picture, bp=base pairs). The strength of the resulting linkages can be tuned by varying the length of DNA strands, allowing for controlled transport of the cells.