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Subdiffraction‐Resolution Fluorescence Imaging with Conventional Fluorescent Probes
Author(s) -
Heilemann Mike,
van de Linde Sebastian,
Schüttpelz Mark,
Kasper Robert,
Seefeldt Britta,
Mukherjee Anindita,
Tinnefeld Philip,
Sauer Markus
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200802376
Subject(s) - fluorescence , resolution (logic) , materials science , wavelength , optics , fluorescence lifetime imaging microscopy , diffraction , nanotechnology , chemistry , optoelectronics , physics , computer science , artificial intelligence
Eagle eyes : dSTORM uses conventional photoswitchable fluorescent dyes that can be reversibly cycled between a fluorescent and a dark state by irradiation with light of different wavelengths (see picture). This elegant approach can visualize cellular structures with a resolution of approximately 20 nm, far beyond the diffraction limit of light, without the need of an activator molecule.