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A Highly Emissive Fluorescent Nucleoside that Signals the Activity of Toxic Ribosome‐Inactivating Proteins
Author(s) -
Srivatsan Seergazhi G.,
Greco Nicholas J.,
Tor Yitzhak
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200802199
Subject(s) - library science , chemistry , computer science
Lethal weapon : The presence of RNA abasic sites generated by ribosome‐inactivating proteins (RIPs) such as ricin can be detected. RNA depurination by toxins is signaled by the enhanced emission intensity of synthetically modified emissive RNA constructs that are complementary to the α‐sarcin/ricin loop (see picture). This method is more efficient and precise than the use of radiolabeled substrates.