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Postsynthetic DNA Modification through the Copper‐Catalyzed Azide–Alkyne Cycloaddition Reaction
Author(s) -
Gramlich Philipp M. E.,
Wirges Christian T.,
Manetto Antonio,
Carell Thomas
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200802077
Subject(s) - cycloaddition , combinatorial chemistry , click chemistry , oligonucleotide , surface modification , chemistry , dna , azide , alkyne , nanotechnology , catalysis , organic chemistry , materials science , biochemistry
The attachment of labels onto DNA is of utmost importance in many areas of biomedical research and is valuable in the construction of DNA‐based functional nanomaterials. The copper(I)‐catalyzed Huisgen cycloaddition of azides and alkynes (CuAAC) has recently been added to the repertoire of DNA labeling methods, thus allowing the virtually unlimited functionalization of both small synthetic oligonucleotides and large gene fragments with unprecedented efficiency. The CuAAC reaction yields the labeled polynucleotides in very high purity after a simple precipitation step. The reviewed technology is currently changing the way in which functionalized DNA strands are generated cost‐efficiently in high quality for their application in molecular diagnostics systems and nanotechnological research.