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Target‐Catalyzed Transfer Reactions for the Amplified Detection of RNA
Author(s) -
Grossmann Tom N.,
Röglin Lars,
Seitz Oliver
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200801355
Subject(s) - rna , flexibility (engineering) , dna , biotin , chemistry , catalysis , computational biology , signal (programming language) , combinatorial chemistry , microbiology and biotechnology , computer science , nanotechnology , biochemistry , biology , gene , materials science , programming language , statistics , mathematics
Strong signals : The RNA‐catalyzed transfer of a biotin reporter was used to preamplify a signal that was detected in an enzyme‐based readout, thereby increasing its sensitivity. The advantages of combining the covalent attachment of biotin and preamplification were demonstrated by the detection of 500 attomol HIV RNA. The flexibility requirements of DNA‐ and RNA‐catalyzed transfer reactions were tested.