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Charge‐Conversion Ternary Polyplex with Endosome Disruption Moiety: A Technique for Efficient and Safe Gene Delivery
Author(s) -
Lee Yan,
Miyata Kanjiro,
Oba Makoto,
Ishii Takehiko,
Fukushima Shigeto,
Han Muri,
Koyama Hiroyuki,
Nishiyama Nobuhiro,
Kataoka Kazunori
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200800963
Subject(s) - moiety , endosome , transfection , cytotoxicity , gene delivery , ternary operation , chemistry , ternary complex , biophysics , stereochemistry , cell , gene , biochemistry , in vitro , biology , computer science , enzyme , programming language
Wrapped for special delivery : A ternary polyplex, with an endosomal disruption moiety based on the charge‐conversion polymer pAsp(DET‐Aco), showed negative charges for serum stability and low cytotoxicity, but the charges became positive and the endosome disruption moiety was exposed (see picture). High transfection efficiency and minimal cytotoxicity were observed with primary cells.