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A Highly Selective DNAzyme Sensor for Mercuric Ions
Author(s) -
Hollenstein Marcel,
Hipolito Christopher,
Lam Curtis,
Dietrich David,
Perrin David M.
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200800960
Subject(s) - deoxyribozyme , mercury (programming language) , divalent , chemistry , nucleobase , cofactor , combinatorial chemistry , ion , metal , enzyme , biochemistry , dna , computer science , organic chemistry , programming language
The mercury project : A self‐cleaving DNAzyme that “switches on” in the presence of Hg 2+ has been revealed by in vitro selection. The enzyme 10‐13 (see picture) utilizes two modified nucleobases to confer an RNaseA‐like catalytic activity that requires only Hg 2+ and no other divalent metal cofactor. This DNAzyme is highly selective for mercuric cations.