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Water‐Based Ligand Screening for Paramagnetic Metalloproteins
Author(s) -
Bertini Ivano,
Fragai Marco,
Luchinat Claudio,
Talluri Eleonora
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200800327
Subject(s) - metalloprotein , paramagnetism , chemistry , relaxation (psychology) , proton , ligand (biochemistry) , nuclear magnetic resonance spectroscopy , metal , nuclear magnetic resonance , physics , stereochemistry , biochemistry , nuclear physics , organic chemistry , condensed matter physics , biology , receptor , neuroscience
The relaxation rate of water proton nuclei can be used to screen ligands (L; see picture) directed to the metal binding site in paramagnetic metalloproteins by NMR spectroscopy. The very high concentration of water protons provides an enormous sensitivity and permits the use of unprecedentedly low amounts of protein.