Premium
Photochemical Surface Patterning by the Thiol‐Ene Reaction
Author(s) -
Jonkheijm Pascal,
Weinrich Dirk,
Köhn Maja,
Engelkamp Hans,
Christianen Peter C. M.,
Kuhlmann Jürgen,
Maan Jan C.,
Nüsse Dirk,
Schroeder Hendrik,
Wacker Ron,
Breinbauer Rolf,
Niemeyer Christof M.,
Waldmann Herbert
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200800101
Subject(s) - chemistry , streptavidin , fluorescence , thiol , phase (matter) , ene reaction , photochemistry , olefin fiber , combinatorial chemistry , organic chemistry , catalysis , biochemistry , biotin , optics , physics
The photochemical coupling of olefin‐capped (bio)molecules to surface‐bound thiols can be used to control protein immobilization on length scales of centimeters to sub‐micrometers (see the fluorescence microscopy image of a nanopattern after treatment with labeled streptavidin). Two enzymes immobilized on the resulting patterns retained their enzymatic activity and underwent protein–protein interactions similar to those in the solution phase.