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Inside Cover: A Nucleoside That Contains a Rigid Nitroxide Spin Label: A Fluorophore in Disguise (Angew. Chem. Int. Ed. 15/2007)
Author(s) -
Barhate Nivrutti,
Cekan Pavol,
Massey Archna P.,
Sigurdsson Snorri Th.
Publication year - 2007
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200790057
Subject(s) - nitroxide mediated radical polymerization , spin label , guanine , nucleoside , dna , chemistry , fluorophore , duplex (building) , nucleic acid , multiplex , biophysics , fluorescence , stereochemistry , biochemistry , physics , nucleotide , bioinformatics , biology , organic chemistry , quantum mechanics , polymer , radical polymerization , membrane , copolymer , gene
A single nucleoside acts as a probe for two complementary spectroscopic techniques. In their Communication on page 2655 ff., S. Th. Sigurdsson and co‐workers describe the synthesis of DNA with a rigid nitroxide spin label. The modified nucleoside forms a stable base pair with guanine and projects the spin label into the major groove of the duplex. The rigidity of the nitroxide enables more accurate measurements of distance and motion in nucleic acids (left), while its reduction yields a fluorescent probe DNA (right).