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Color Change of Proteorhodopsin by a Single Amino Acid Replacement at a Distant Cytoplasmic Loop
Author(s) -
Yoshitsugu Maiko,
Shibata Mikihiro,
Ikeda Daisuke,
Furutani Yuji,
Kandori Hideki
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200705989
Subject(s) - rhodopsin , chromophore , loop (graph theory) , graph , cytoplasm , pigment , amino acid , nirenberg and matthaei experiment , biology , chemistry , biochemistry , genetics , retinal , photochemistry , combinatorics , mathematics , organic chemistry
Switching from red to pink : It has been believed that only the amino acids near the retinal chromophore are responsible for the color tuning of rhodopsins. Herein is reported a color change of proteorhodopsin, an archaeal‐type rhodopsin in marine bacteria, by mutation of Ala178 to Arg at the E–F loop. The red shift at a neutral pH value amounts to 20 nm (see graph).