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Mass Spectrometric Method for Analyzing Metabolites in Yeast with Single Cell Sensitivity
Author(s) -
Amantonico Andrea,
Oh Joo Yeon,
Sobek Jens,
Heinemann Matthias,
Zenobi Renato
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200705923
Subject(s) - metabolomics , mass spectrometry , yeast , chromatography , detection limit , chemistry , sensitivity (control systems) , cell , metabolite , endogeny , single cell analysis , biochemistry , engineering , electronic engineering
Getting a look‐in : An optimized MALDI‐MS procedure has been developed to detect endogenous primary metabolites directly in the cell extract. A detection limit corresponding to metabolites from less than a single cell has been attained, opening the door to single‐cell metabolomics by mass spectrometry.