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Synthesis of Protein Mimics with Nonlinear Backbone Topology by a Combined Recombinant, Enzymatic, and Chemical Synthesis Strategy
Author(s) -
Pritz Stephan,
Kraetke Oliver,
Klose Annerose,
Klose Jana,
Rothemund Sven,
Fechner Klaus,
Bienert Michael,
Beyermann Michael
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200705718
Subject(s) - recombinant dna , ligand (biochemistry) , enzyme , sortase a , extracellular , chemistry , ligation , receptor , biochemistry , combinatorial chemistry , computational biology , topology (electrical circuits) , biology , bacterial protein , microbiology and biotechnology , gene , engineering , electrical engineering
The synthesis of a 23‐kDa protein that mimics the ligand‐binding extracellular part of a G‐protein‐coupled receptor shows the potential of a combined recombinant, enzymatic, and chemical synthesis (CRECS) strategy. The mimic of the corticotropin‐releasing factor receptor, synthesized from single domains by chemical ligation and sortase A‐mediated coupling, has a high affinity for natural ligands.