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Chromophore Heterogeneity and Photoconversion in Phytochrome Crystals and Solution Studied by Resonance Raman Spectroscopy
Author(s) -
von Stetten David,
Günther Mina,
Scheerer Patrick,
Murgida Daniel H.,
Mroginski Maria Andrea,
Krauß Norbert,
Lamparter Tilman,
Zhang Junrui,
Anstrom David M.,
Vierstra Richard D.,
Forest Katrina T.,
Hildebrandt Peter
Publication year - 2008
Publication title -
angewandte chemie international edition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.831
H-Index - 550
eISSN - 1521-3773
pISSN - 1433-7851
DOI - 10.1002/anie.200705716
Subject(s) - phytochrome , chromophore , raman spectroscopy , resonance raman spectroscopy , spectroscopy , deinococcus radiodurans , resonance (particle physics) , chemistry , photochemistry , materials science , biophysics , optics , physics , biology , biochemistry , botany , atomic physics , red light , quantum mechanics , gene
Crystals of the photoreceptor phytochrome were studied nondestructively by resonance Raman spectroscopy. The spectra of the chromophore‐binding domains (CBD) of phytochrome from Deinococcus radiodurans , from which three‐dimensional structures have been determined, demonstrate the same chromophore structure in the parent Pr state as in solution. Unlike CBD, crystals of phytochrome from Agrobacterium tumefaciens including the CBD and the PHY domain can be photoconverted to a Meta‐Rc‐like state.